Description
| Product Name: | Transglutaminases (TG) Activity Colorimetric Assay Kit |
| Product Code: | MAES0470 |
| Product Size: | 96T (40 samples) |
| Detection Method: | Colorimetric method |
| Sample Volume: | 120 μL (60 μL each for the sample and control tubes) |
| Assay Time: | 1 h 40 min |
| Instrument: | Microplate reader (520-530 nm, optimal 525 nm) |
| Sensitivity: | 3.0 U/L |
| Detection Range: | 3.0-25.0 U/L |
| Recovery Rate: | 102.7% |
| Intra-assay CV: | 2.0-2.9% |
| Inter-assay CV: | 5.0-6.1% |
Transglutaminases (TGs) catalyse the transfer of acyl groups between glutamine residues and lysine residues or primary amines. TG-mediated protein crosslinking is essential for a variety of biological processes and diseases. TGs are also widely used as a food processing aid in meat, fish, dairy and grain products.
TGs catalyse the acyl transfer between glutamine residues and primary amines. The product forms a chromogenic substance with the chromogenic agent, and the activity of TGs in the sample is determined by measuring the absorbance change at 525 nm.
| Shelf Life: | 12 Months |
| Storage: | -20°C, protected from light |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Research Area: | Amino Acids and Proteins |
| Research Use: | For Research Use Only. Not for use in clinical diagnosis. |
