T-Cell Activation (TCR) Antibody Sampler Pack

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SKU:
ABSP023
Product Type:
Antibody
Size:
Antibody Sampler Pack
Size:
8 X 20μL
Reactivity:
Human
Mouse
Rat
Analytes:
CD3
CD28
Lck
ZAP-70
LAT
PLC-gamma1
NFAT
CD69
  • Anti-CD3E Antigen Antibody (CAB12415)
  • Anti-CD28 [ARC5082-01] Monoclonal Antibody (CAB20346)
  • Anti-LCK Antibody (CAB2177)
  • Anti-ZAP70 [ARC51229] Monoclonal Antibody (CAB9536)
  • Anti-LAT Antibody (CAB5650)
  • Anti-PLC gamma 1 (PLCG1) [ARC1345] Monoclonal Antibody (CAB8899)
  • Anti-NFAT2 [ARC57041] Monoclonal Antibody (CAB23485)
  • Anti-CD69 [ARC56158] Monoclonal Antibody (CAB22171)

Description

T-Cell Activation (TCR) Antibody Sampler Pack

The T-Cell Activation (TCR) Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ELISA, FC, IF/ICC, IHC-P, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.

TargetAntibodyCat. No.HostReactivityApplicationsSize
CD3Anti-CD3E Antigen AntibodyCAB12415-20RabbitHuman, Mouse, RatWBIHC-PIF/ICCELISA20µL
CD28Anti-CD28 [ARC5082-01] Monoclonal AntibodyCAB20346-20RabbitHuman, Mouse, RatWBFCELISA20µL
LckAnti-LCK AntibodyCAB2177-20RabbitHuman, Mouse, RatWBIHC-PELISA20µL
ZAP-70Anti-ZAP70 [ARC51229] Monoclonal AntibodyCAB9536-20RabbitHuman, MouseWBELISA20µL
LATAnti-LAT AntibodyCAB5650-20RabbitHumanWBELISA20µL
PLC-gamma1Anti-PLC gamma 1 (PLCG1) [ARC1345] Monoclonal AntibodyCAB8899-20RabbitHuman, Mouse, RatWBELISA20µL
NFATAnti-NFAT2 [ARC57041] Monoclonal AntibodyCAB23485-20RabbitHuman, MouseWBELISA20µL
CD69Anti-CD69 [ARC56158] Monoclonal AntibodyCAB22171-20RabbitMouse, RatWBIF/ICCELISA20µL

T-cell activation is the central event in adaptive immunity, converting antigen recognition into clonal expansion and effector differentiation. The process begins when the T-cell receptor (TCR) engages a peptide-MHC complex on an antigen-presenting cell. The TCR itself lacks intrinsic catalytic activity and signals through the associated CD3 complex, whose immunoreceptor tyrosine-based activation motifs (ITAMs) become phosphorylated by the Src-family kinase Lck, which is recruited via the CD4 or CD8 coreceptor. Phosphorylated CD3 ITAMs create docking sites for the tyrosine kinase ZAP-70, which is itself activated by Lck and then phosphorylates downstream scaffolds. Foremost among these is the transmembrane adaptor LAT, whose phosphorylation nucleates a signaling complex that recruits and activates phospholipase C-gamma1 (PLC-gamma1). PLC-gamma1 hydrolyzes membrane phosphoinositides to generate diacylglycerol and inositol trisphosphate, triggering calcium flux and protein kinase C activation. The resulting calcium signal activates calcineurin, which dephosphorylates the transcription factor NFAT and permits its nuclear translocation to drive cytokine gene expression, including IL-2. Full, productive activation requires a second signal delivered through the costimulatory receptor CD28, which engages B7 ligands and amplifies and sustains signaling; in its absence, T cells become anergic. The early activation marker CD69 is rapidly induced upon stimulation and serves as a reliable readout of T-cell engagement and tissue retention. Defects or dysregulation in these proximal signaling events underlie immunodeficiencies, autoimmunity, and lymphoid malignancies, and the pathway is the basis for many immunomodulatory therapies. Probing the proximal signalosome requires reagents spanning receptors, kinases, adaptors, and transcription factors. This sampler pack brings together validated antibodies against CD3, CD28, Lck, ZAP-70, LAT, PLC-gamma1, NFAT, and CD69 for studying proximal TCR signaling and T-cell activation.

AnalyteAlternate Names
CD3CD3D, CD3E, CD3G, T-cell receptor complex
CD28T-cell-specific surface glycoprotein CD28, Tp44
LckLCK, Lymphocyte cell-specific protein-tyrosine kinase, p56lck
ZAP-70Zeta-chain-associated protein kinase 70, SRK, TZK, STD
LATLinker for activation of T-cells, p36-38, LAT1
PLC-gamma1PLCG1, Phospholipase C-gamma-1, PLC-148, PLC1
NFATNFATC1, NFAT2, Nuclear factor of activated T-cells, NFATc
CD69Early activation antigen CD69, AIM, EA1, CLEC2C
SpecificationDetail
Pack Format8 antibodies, 20µL each (trial size)
StorageStore at -20°C upon receipt
Shelf Life12 months from date of receipt
ShippingGel pack
Scale-upEvery antibody is also available individually in a full 100µL size

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