Rat SCD86 (Soluble Cluster of Differentiation 41 Ligand) ELISA Kit
The Rat SCD86 (Soluble Cluster of Differentiation 86) ELISA Kit is specially designed for the precise quantification of soluble cluster of differentiation 86 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Soluble cluster of differentiation 86, also known as CD86, is a key protein involved in immune response regulation and T-cell activation. It plays a crucial role in modulating immune responses and is implicated in autoimmune diseases, allergy, and transplant rejection. The Rat SCD86 ELISA Kit provides a valuable tool for studying the role of CD86 in these conditions and exploring potential therapeutic interventions.Overall, the Rat SCD86 ELISA Kit is an indispensable tool for researchers investigating the immune system, inflammatory disorders, and autoimmune diseases in rat models. Its reliability and precision make it an essential addition to any laboratory conducting research in these areas.
Product Name:
Rat SCD86 (Soluble Cluster of Differentiation 41 Ligand) ELISA Kit
SKU:
RTES00743
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
