Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit
The Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is a highly reliable and accurate tool for the quantitative measurement of sCD40L levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.sCD40L is a key protein involved in immune response regulation and inflammation, playing a critical role in various diseases including autoimmune disorders, cardiovascular diseases, and infectious conditions. By accurately measuring sCD40L levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.The Human sCD40L ELISA Kit from Assay Genie provides a convenient and efficient solution for studying the role of sCD40L in health and disease. With easy-to-follow protocols and high-quality components, this kit is an essential tool for any laboratory conducting research in immunology, inflammation, and related fields.
Product Name:
Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit
SKU:
HUES01322
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
