Human ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit
The Human ENA-78 (Epithelial Neutrophil Activating Peptide-78) ELISA Kit is a cutting-edge tool designed for the precise detection of ENA-78 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results to support a wide range of research endeavors.ENA-78, also known as CXCL5, is a key chemokine involved in inflammatory processes, particularly in recruiting neutrophils to sites of infection or injury. Its dysregulation has been implicated in various diseases like asthma, COPD, and autoimmune disorders, highlighting its value as a biomarker for understanding and potentially treating these conditions.With the Human ENA-78 ELISA Kit, researchers can delve deeper into the role of this potent chemokine in health and disease, paving the way for innovative therapeutic interventions and clinical advancements.
Product Name:
Human ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit
SKU:
HUES01333
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 2 vials, 20 mL | 96T*5: 10 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
94-107
99-111
96-113
Average (%)
99
105
103
1:4
Range (%)
92-103
87-100
86-99
Average (%)
98
92
92
1:8
Range (%)
92-106
83-96
88-99
Average (%)
98
90
94
1:16
Range (%)
91-103
85-95
87-100
Average (%)
97
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
88-102
93
Cell Culture Media (n=8)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.78
177.05
709.29
92.4
180.55
759.1
Standard deviation
6.71
8.99
43.41
6.23
16.0
52.99
C V (%)
6.93
5.08
6.12
6.74
8.86
6.98
Uniport ID:
P42830
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human ENA-78/CXCL5 in samples. No significant cross-reactivity or interference between Human ENA-78/CXCL5 and analogues was observed
