Human ACY1 Recombinant Protein (RPPB5783)
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주문- SKU:
- RPPB5783
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- ACY1
- Synonyms:
- N-acyl-L-amino-acid amidohydrolase
- Synonyms:
- ACY-1
- Synonyms:
- ACY1D
- Synonyms:
- ACYLASE
- Source:
- Escherichia Coli
- Uniprot:
- Q03154
Description
| Product Name: | Human ACY1 Recombinant Protein |
| Product Code: | RPPB5783 |
| Size: | 10µg |
| Species: | Human |
| Target: | ACY1 |
| Synonyms: | N-acyl-L-amino-acid amidohydrolase, ACY-1, ACY1D, ACYLASE, ACY1,EC 3.5.1.143. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The ACY1 solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MTSKGPEEEH PSVTLFRQYL RIRTVQPKPD YGAAVAFFEE TARQLGLGCQ KVEVAPGYVV TVLTWPGTNP TLSSILLNSH TDVVPVFKEH WSHDPFEAFK DSEGYIYARG AQDMKCVSIQ YLEAVRRLKV EGHRFPRTIH MTFVPDEEVG GHQGMELFVQ RPEFHALRAG FALDEGIANP TDAFTVFYSE RSPWWVRVTS TGRPGHASRF MEDTAAEKLH KVVNSILAFR EKEWQRLQSN PHLKEGSVTS VNLTKLEGGV AYNVIPATMS ASFDFRVAPD VDFKAFEEQL QSWCQAAGEG VTLEFAQKWM HPQVTPTDDS NPWWAAFSRV CKDMNLTLEP EIMPAATDNR YIRAVGVPAL GFSPMNRTPV LLHDHDERLH EAVFLRGVDI YTRLLPALAS VPALPSDS |
Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.
ACY1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 428 amino acids (1-408 a.a.) and having a molecular mass of 48kDa. The ACY1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ACY1: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. Belongs to the peptidase M20A family. |
| UniProt Protein Details: | Protein type:Hydrolase; EC 3.5.1.14; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: cytosol Molecular Function:metallopeptidase activity; metal ion binding; aminoacylase activity Biological Process: amino acid metabolic process; xenobiotic metabolic process; proteolysis Disease: Aminoacylase 1 Deficiency |
| NCBI Summary: | This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010] |
| UniProt Code: | Q03154 |
| NCBI GenInfo Identifier: | 461466 |
| NCBI Gene ID: | 95 |
| NCBI Accession: | Q03154.1 |
| UniProt Secondary Accession: | Q03154,C9J6I6, C9J9D8, C9JWD4, |
| UniProt Related Accession: | Q03154 |
| Molecular Weight: | 408 |
| NCBI Full Name: | Aminoacylase-1 |
| NCBI Synonym Full Names: | aminoacylase 1 |
| NCBI Official Symbol: | ACY1 |
| NCBI Official Synonym Symbols: | ACY-1; ACY1D; HEL-S-5 |
| NCBI Protein Information: | aminoacylase-1; epididymis secretory protein Li 5; N-acyl-L-amino-acid amidohydrolase |
| UniProt Protein Name: | Aminoacylase-1 |
| UniProt Synonym Protein Names: | N-acyl-L-amino-acid amidohydrolase |
| Protein Family: | Aminoacylase |
| UniProt Gene Name: | ACY1 |
| UniProt Entry Name: | ACY1_HUMAN |
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