DNA Damage Response Antibody Sampler Pack

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SKU:
ABSP008
Product Type:
Antibody
Size:
Antibody Sampler Pack
Size:
8 X 20μL
Reactivity:
Human
Mouse
Rat
Analytes:
ATM
Phospho-ATM
ATR
Chk1
Chk2
gamma-H2AX
BRCA1
53BP1
  • Anti-ATM [ARC0129] Monoclonal Antibody (CAB19650)
  • Anti-Phospho-ATM-S1981 [ARC1575] Monoclonal Antibody (CABP1030)
  • Anti-ATR [ARC51749] Monoclonal Antibody (CAB21253)
  • Anti-Chk1 Monoclonal Antibody (CAB22055)
  • Anti-Chk2 Monoclonal Antibody (CAB19543)
  • Anti-Phospho-Histone H2AX-S139 [ARC0110] Monoclonal Antibody (CABP0687)
  • Anti-BRCA1 Antibody (CAB11549)
  • Anti-TP53BP1 Polyclonal Antibody (CAB21632)

Description

DNA Damage Response Antibody Sampler Pack

The DNA Damage Response Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ELISA, IF/ICC, IHC-P, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.

TargetAntibodyCat. No.HostReactivityApplicationsSize
ATMAnti-ATM [ARC0129] Monoclonal AntibodyCAB19650-20RabbitHuman, MouseWBIHC-PELISA20µL
Phospho-ATMAnti-Phospho-ATM-S1981 [ARC1575] Monoclonal AntibodyCABP1030-20RabbitHumanWBELISA20µL
ATRAnti-ATR [ARC51749] Monoclonal AntibodyCAB21253-20RabbitHuman, MouseWBELISA20µL
Chk1Anti-Chk1 Monoclonal AntibodyCAB22055-20RabbitHumanWBELISA20µL
Chk2Anti-Chk2 Monoclonal AntibodyCAB19543-20RabbitHuman, MouseWBIHC-PELISA20µL
gamma-H2AXAnti-Phospho-Histone H2AX-S139 [ARC0110] Monoclonal AntibodyCABP0687-20RabbitHuman, Mouse, RatWBIHC-PIF/ICCELISA20µL
BRCA1Anti-BRCA1 AntibodyCAB11549-20RabbitHumanIHC-PIF/ICCELISA20µL
53BP1Anti-TP53BP1 Polyclonal AntibodyCAB21632-20RabbitHuman, Mouse, RatWBIHC-PELISA20µL

The DNA damage response is a coordinated signaling network that detects genomic lesions, halts cell-cycle progression, and orchestrates repair, thereby safeguarding genome integrity. The response is anchored by two apical kinases: ATM, which is activated chiefly by DNA double-strand breaks and whose autophosphorylation is detected as Phospho-ATM, and ATR, which responds primarily to single-stranded DNA and replication stress. These kinases phosphorylate a cascade of effectors to transmit and amplify the damage signal. One of the earliest events at double-strand breaks is phosphorylation of the histone variant H2AX to generate gamma-H2AX, which spreads across megabase domains flanking the lesion and serves as a platform for recruiting repair and signaling factors. The mediator 53BP1 accumulates at these sites and influences the choice between repair pathways, while the tumor suppressor BRCA1 promotes high-fidelity homologous recombination. The transducer kinases Chk1, activated principally downstream of ATR, and Chk2, activated downstream of ATM, propagate checkpoint signaling to arrest the cell cycle and stabilize stalled replication forks, providing time for repair or, when damage is irreparable, triggering apoptosis or senescence. Defects in this network are central to carcinogenesis: loss of ATM, BRCA1, or checkpoint function generates genomic instability that fuels tumor evolution, yet these same deficiencies create therapeutic vulnerabilities, most prominently the synthetic-lethal sensitivity of BRCA1-deficient tumors to PARP inhibitors and the targeting of ATR and Chk1 in replication-stressed cancers. Quantifying the activation and recruitment of these factors provides a mechanistic readout of damage burden, checkpoint engagement, and repair capacity. This pack brings together validated antibodies against ATM, Phospho-ATM, ATR, Chk1, Chk2, gamma-H2AX, BRCA1, and 53BP1 for studying the DNA damage response.

AnalyteAlternate Names
ATMAtaxia telangiectasia mutated, ATM serine/threonine kinase
Phospho-ATMATM, Ataxia telangiectasia mutated (phospho-Ser1981)
ATRATR serine/threonine kinase, FRP1, ataxia telangiectasia and Rad3-related
Chk1CHEK1, Checkpoint kinase 1, CHK1
Chk2CHEK2, Checkpoint kinase 2, RAD53, HuCds1
gamma-H2AXH2AX, H2AFX, Histone H2AX (phospho-Ser139)
BRCA1Breast cancer type 1 susceptibility protein, RNF53, BRCC1
53BP1TP53BP1, p53-binding protein 1
SpecificationDetail
Pack Format8 antibodies, 20µL each (trial size)
StorageStore at -20°C upon receipt
Shelf Life12 months from date of receipt
ShippingGel pack
Scale-upEvery antibody is also available individually in a full 100µL size

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