Boron Assay Kit (BA0212)

Product Name:	Boron Assay Kit (BA0212)
SKU:	BA0212
Detection Method:	Colorimetric (OD 420 nm)
Detection Range:	0.05 - 10 ug/mL (0.05 - 10 ppm) boron with 100 uL sample (96-well)
Sample Type:	Water, plant tissues, soil samples and antibody conjugation solutions
Species Reactivity:	All
Assay Time:	40 minutes
Kit Size:	100 Assays
Equipment Required:	Microplate reader
Storage:	Shipped at room temperature. Store all components at 2-8 degrees C upon receipt.
Shelf Life:	12 months after receipt
Shipping:	Room Temperature

Boron is an essential micronutrient in plants and is involved in maintaining robust cell walls, cell membranes and reproductive tissues. Although boron is common in the soil in its natural state as a borate mineral, the amount available to plants is quite small, and boron deficiency is the second most common micronutrient deficiency among crop plants. Supplementation to the soil via fertilisers and additives is often required, as a lack of or excess of boron may significantly lower crop yield. In the biotech industry, sodium borohydride is commonly used to conjugate antibodies and typically needs to be removed from the final product, especially for therapeutic antibodies. This detection kit provides a convenient and reliable means to measure boron. In the assay, borate complexes with azomethine-H to create a coloured compound that can be measured at 420 nm. The assay can be used with a variety of samples and is simple, sensitive and adaptable to high-throughput screening.

Fast and sensitive. Linear detection range: 0.05 to 10 ug/mL (0.05-10 ppm) boron with 100 uL sample (96-well).
Convenient. The procedure involves adding a single working reagent.
High-throughput. Add-mix-read type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.

Direct assays of boron in water, plant tissues and soil samples.
Direct assays of boron in antibody conjugation solutions.

Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Prepare 500 uL of 10 ug/mL Premix by mixing 10 uL of the 500 ug/mL Standard and 490 uL of water, then dilute the standards in 1.5-mL centrifuge tubes as described in the standard table (10, 6, 3 and 0 ug/mL).
2	Transfer 100 uL of each standard into separate wells of a clear, flat-bottom 96-well plate and transfer 100 uL of each sample into two separate wells (one Sample well and one Sample Background well).
3	Add 150 uL of water to all Sample Background wells.
4	Add 250 uL of water to a separate well on the plate (Water Blank well).
5	Prepare the Working Reagent (WR) fresh and use within 15 minutes of reconstitution; for each standard and sample well, weigh out 2 mg of Reagent A powder and dissolve in 160 uL of Reagent B.
6	Add 150 uL of WR to each standard and sample well, tap the plate lightly to ensure the well contents are mixed evenly, and incubate for 40 minutes at room temperature, protected from light.
7	Read the optical density at 420 nm.

Subtract the blank value (Standard #4) from the standard values and plot the delta-OD against standard concentrations. Determine the slope and calculate the boron concentration of a sample as: [Boron] = [((OD_SAMPLE - OD_BLANK) - (OD_SAMPLE BG - OD_H2O)) / Slope (per ug/mL)] x n (ug/mL), where OD_SAMPLE, OD_SAMPLE BG and OD_BLANK are the optical density readings of the sample, sample background and blank (Standard #4) respectively, and OD_H2O is the OD of the Water Blank well. If the calculated boron concentration is higher than 10 ug/mL, dilute the sample in water and repeat the assay, then multiply the result by the dilution factor n. Conversions: 1 ug/mL boron equals 1 ppm or 92.5 uM.

Component	Quantity	Storage
Reagent A	250 mg	2-8 degrees C
Reagent B	20 mL	2-8 degrees C
Standard (500 ug/mL boron)	150 uL	2-8 degrees C

Boron Assay Kit (BA0212)

Description