Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody

Product Name:	Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0091
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	RB6-8C5
Protein:	Ly-6G/Ly-6C
Product Type:	Monoclonal Antibody
Synonyms:	Gr-1
Isotype:	Rat IgG2b
Reactivity:	Mouse
Immunogen:	Raised against granulocytes of mouse origin
Applications:	CyTOF®, Depletion, IHC FF, IP, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0091	Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low Endotoxin	Low
IVMB0092	Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	CyTOF®, Depletion, IHC FF, IP, WB
Recommended Usage:	FC The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 µg per 10⁶ in a volume of 100 µl or 100 µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Rat Anti-Mouse GR-1, as known as anti-mouse Ly-6G/Ly-6C (Clone RB6-8C5) has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:	The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2
Immunogen:	Raised against granulocytes of mouse origin
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

Technical Datasheet:	View
Protein:	Ly-6G/Ly-6C
Research Area:	Immunology, Innate Immunity

Rat IgG2b Isotype Control
Clone	1-2
Isotype	Rat IgG2b
Endotoxin Level	Low Endotoxin

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