Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0177
Product Type:
In Vivo Monoclonal Antibody
Clone:
HK1.4
Protein:
Ly-6C
Isotype:
Rat IgG2c
Reactivity:
Mouse
Synonyms:
Lymphocyte antigen 6 complex
Synonyms:
locus C
Research Area:
MHC & Immune Cell-Specific
Endotoxin Level:
Low Endotoxin
Host Species:
Rat
Applications:
CODEX
Applications:
CyTOF
Applications:
IHC FF
Applications:
IP
Applications:
WB
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Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin
Product Code:IVMB0177
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:HK1.4
Protein:Ly-6C
Product Type:Monoclonal Antibody
Synonyms:Lymphocyte antigen 6 complex, locus C
Isotype:Rat IgG2c
Reactivity:Mouse
Immunogen:L3 cloned CTL cells
Applications:CyTOF®, IHC FF, IP, PhenoCycler®, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:CODEX®, CyTOF®, IHC FF, IP, WB
Reactivity:Mouse
Host Species:Rat
Specificity:Rat Anti-Mouse Ly-6C (Clone HK1.4) recognizes an epitope on Mouse Ly-6C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5
Immunogen:L3 cloned CTL cells
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Technical Datasheet:View
Protein:Ly-6C
Research Area:Immunology
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