The ACAT1 Polyclonal Antibody (CAB5335) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the ACAT1 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in metabolic disorders, cardiovascular diseases, and lipid metabolism research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACAT1 Polyclonal Antibody
SKU:
CAB5335
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T2, MAT, ACAT, THIL, ACAT1
Positive Sample:
PC-3, DU145, A-549, RAW264.7, Neuro-2a, Mouse heart, Rat heart, Rat liver，Rat kidney
Cellular Localization:
Mitochondrion.
Calculated MW:
45kDa
Observed MW:
42kDa
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Purification Method
Affinity purification
Gene ID
38
RRID
AB_2766146
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ACAT1 Rabbit pAb (CAB5335) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
