The ACAT1 Antibody (CAB13273) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ACAT1 protein, this antibody allows for the detection and analysis of ACAT1 levels in various cell types, making it an excellent choice for studies in biochemistry and lipid metabolism research.ACAT1, also known as acyl-CoA:cholesterol acyltransferase 1, plays a crucial role in the esterification of cholesterol, a process essential for the storage and transport of this lipid in cells.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACAT1 Antibody
SKU:
CAB13273
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T2, MAT, ACAT, THIL, ACAT1
Positive Sample:
PC-3, DU145, A-549, RAW264.7, Neuro-2a, Mouse heart, Rat heart, Rat liver, Rat kidney
Cellular Localization:
Mitochondrion.
Calculated MW:
45kDa
Observed MW:
42kDa
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Purification Method
Affinity purification
Gene ID
38
RRID
AB_2760125
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ACAT1 Rabbit pAb (CAB13273) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ACAT1 Rabbit pAb (CAB13273) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using ACAT1 Rabbit pAb (CAB13273) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
