Description
Zaire Ebola virus Nucleoprotein (NP) Human IgM ELISA Kit
| Product SKU: | SBRS8850 |
| Size: | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species: | Human, Ebolavirus |
| Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Method of Detection: | Colorimetric |
| Component | Quantity |
|---|---|
| Pathogen antigen coated 96-well Microplate | 12 strips x 8 wells |
| Wash Buffer Concentrate (20X) | 25 ml |
| Positive Control | 1 vial |
| Biotinylated Anti-Human Ig(A/G/M) | 1 vial |
| Biotinylated Anti-Positive Control | 1 vial |
| HRP-Streptavidin concentrate | 1 vial |
| TMB One-Step Substrate Reagent | 12 ml |
| Stop Solution | 8 ml |
| Assay Diluent B | 15 ml |
| Sample Diluent (5X) | 25 ml |
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control, or sample to each well.
- Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated Anti-Positive Control and Biotinylated Anti-Human Ig(A/G/M) Antibody into respective wells.
- Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well.
- Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well.
- Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.
