The UBE2E2 Antibody (CAB16441) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable choice for Western blot applications. By targeting the UBE2E2 protein, researchers can investigate its function and role in various cellular pathways.UBE2E2 is a member of the ubiquitin-conjugating enzyme family and plays a crucial role in the transfer of ubiquitin molecules to target proteins, marking them for degradation or altering their function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
UBE2E2 Antibody
SKU:
CAB16441
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UBCH8, UBE2E2
Positive Sample:
Rat testis, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
22kDa
Observed MW:
22kDa
Enables ISG15 transferase activity and ubiquitin conjugating enzyme activity. Involved in protein modification by small protein conjugation. Acts upstream of or within cellular response to DNA damage stimulus and positive regulation of G1/S transition of mitotic cell cycle. Predicted to be active in nucleus.
Purification Method
Affinity purification
Gene ID
7325
RRID
AB_2772776
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UBE2E2 Rabbit pAb (CAB16441) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
