KMT5A/SETD8 Rabbit Polyclonal Antibody (CAB5256)

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SKU:
CAB5256
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
SET8, SET07, SETD8, PR-Set7, PR/SET07, KMT5A/SETD8
Research Use:
Cell Cycle

Description

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KMT5A/SETD8 Antibody (CAB5256)

The KMT5A/SETD8 Antibody (CAB5256) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for use in Western blotting and immunohistochemistry applications.SETD8 is known to play a critical role in gene expression regulation and chromatin organization, making it a key player in epigenetic mechanisms that influence cellular function and development.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:KMT5A/SETD8 Antibody
SKU:CAB5256
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGEG GAAA ALVA AAAA AAAA AAAV VAGQ RRRR LGRR ARCH GPGR AAGG KMSK PCAV EAAA AAVA ATAP GPEM VERR GPGR PRTD GENV FTGQ SKIY SYMS PNKC SGMR FPLQ EENS VTHH EVKC QGKP LAGI YRKR EEKR NAGN AVRS AMKS EEQK IKDA RKGP LVPF PNQK SEAA EPPK TPPS SCDS TNAA IAKQ ALKK PIKG KQAP RKKA QGKT QQNR KLTD FYPV RRSS RKSK AELQ SEER KRID ELIE SGKE EGMK IDLI DGKG RGVI ATKQ FSRG DFVV EYHG DLIE ITDA KKRE ALYA QDPS TGCY MYYF QYLS KTYC VDAT RETN RLGR LINH SKCG NCQT KLHD IDGV PHLI LIAS RDIA AGEE LLYD YGDR SKAS IEAH PWLK H
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SET8, SET07, SETD8, PR-Set7, PR/SET07, KMT5A/SETD8
Positive Sample:HeLa, Jurkat, U-87MG, Mouse heart, Rat lung
Cellular Localization:Chromosome, Nucleus.
Calculated MW:43kDa
Observed MW:45kDa

The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.

Purification MethodAffinity purification
Gene ID387893
RRIDAB_2766083
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using (CAB5256) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of various lysates using (CAB5256) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using KMT5A/KMT5A/SETD8 Rabbit pAb (CAB5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using KMT5A/KMT5A/SETD8 Rabbit pAb (CAB5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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