Description
| SKU: | RPCB2694 |
| Size: | 10 μg, 20 μg, 50 μg, 100 μg |
| Tag: | C-hFC |
| Reactivity: | Human |
| Protein Description: | Recombinant Human Wnt-3a Protein is produced by HEK293 cells expression system. The target protein is fused with a hFc tag at the C-terminus. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
 | Recombinant Human Wnt-3a Surrogate Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. Recombinant Human Wnt-3a Surrogate Protein induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 3. 95-15. |
 | 79 ng/mL, corresponding to a specific activity of 6. 33×10 4~2. 53×10 5 units/mg. |
