Recombinant Human Leukemia inhibitory factor/LIF Protein
The Recombinant Human Leukemia inhibitory factor/LIF Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Leukemia inhibitory factor/LIF function and its implications in health and disease.
This product (SKU: RPCB1058) is produced using HEK293 cells and features a NO-tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 19.72 kDa with an observed molecular weight of 30-45 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Leukemia inhibitory factor/LIF Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Leukemia inhibitory factor/LIF Protein (RPCB1058), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED 50 for this effect is 0.14-0.54 ng/mL, corresponding to a specific activity of 1.85×10 6 -7.14×10 6 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells.
Recombinant Human Leukemia inhibitory factor/LIF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human LIF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 for this effect is 0.14-0.54 ng/mL, corresponding to a specific activity of 1.85×106-7.14×106 units/mg.
