Recombinant Human Intrinsic factor/CBLIF Protein (RPCB1831)
- SKU:
- RPCB1831
- Size:
- 10µg
- Tag:
- C-His
- Reactivity:
- Human
- Expression Host:
- HEK293 Cells
Description
| Product Name: | Recombinant Human Intrinsic factor/CBLIF Protein |
| SKU: | RPCB1831 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Human |
| Expression Host: | HEK293 Cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Intrinsic factor/CBLIF Protein , tested reactivity in HEK293 Cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.01EU/μg |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 2694 |
Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P27352-1 |
 | Recombinant Human Intrinsic factor/CBLIF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-60 kDa. |
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