The Recombinant Human IFN-gamma Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IFN-gamma function and its implications in health and disease.
This product (SKU: RPCB0768) is produced using advanced expression systems and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 16.78 kDa with an observed molecular weight of 17 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IFN-gamma Protein (RPCB0768), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1.97 ng/mL.
Measured in a cytotoxicity assay using HT-29 cells. The ED 50 for this effect is 0.74-2.96 ng/mL, corresponding to a specific activity of 3.38×10 5 ~1.35×10 6 units/mg.
Flow cytometry: 1X10 6 A549 cells treated with IFN-γ (RPCB0768,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692,5 μl/Test,right).
Flow cytometry: 1X10 6 NCI-H460 cells treated with IFN-γ (RPCB0768,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692,5 μl/Test,right)
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Interferon-gamma (IFN-gamma ) is a secreted protein which belongs to the type II interferon family.Recombinant Human IFN-gamma is a 16.8 kDa protein containing 143 amino acid residues. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. Interferon-gamma is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway.
Recombinant Human IFN-gamma Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Recombinant Human IFN-gamma Protein is greater than 95% as determined by SEC-HPLC.
Recombinant Human IFN-gamma induces cytotoxicity in the HT-29 cells. The ED50 for this effect is 0.74-2.96 ng/mL, corresponding to a specific activity of 3.38×105~1.35×106 units/mg.
Flow cytometry: 1X106 A549 cells treated with IFN-γ (RPCB0768,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692,5 μl/Test,right)
