Description
Recombinant human Ephrin-A3/EFNA3 Protein
The Recombinant human Ephrin-A3/EFNA3 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB1185) is produced using HEK293 cells and features a C-6His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 22.33 kDa with an observed molecular weight of 35-45 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant human Ephrin-A3/EFNA3 Protein |
| SKU: | RPCB1185 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | human |
| Synonyms: | EFL2, EPLG3, LERK3, Ehk1-L, EFNA3 |
| Tag: | C-6His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 22.33 kDa |
| Observed MW: | 35-45 kDa |
| Gene ID: | 1944 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant human Ephrin-A3/EFNA3 Protein (RPCB1185), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.01 EU/μg of the protein by LAL method |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Human EFNA3(RPCB1185) at 2 μg/mL (100 μL/well) can bind Human EphA3 (RPCB0285) with a linear range of 0.02-2.82 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Ephrin-A3 (Ephrin A3) is also known as EFL-2, EHK1 ligand, EHK1-L, EPH-related receptor tyrosine kinase ligand 3, EFL2, EPLG3 and LERK3,which comprises the largest subfamily of receptor protein-tyrosine kinases (RTKs), and has been involved in a variety of biological processes, especially in the nervous system and in erythropoiesis, such as axon guidance and topographic map formation, synaptic plasticity, angiogenesis, and meanwhile have possible contributions to tumor growth and metastasis. Ephrin A3 is cell surface GPI-bound ligand for Eph receptors and belongs to the family of receptor tyrosine kinases. Ephrin can bind promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells.
 | Recombinant human Ephrin-A3/EFNA3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized Human EFNA3(RPCB1185) at 2 μg/mL (100 μL/well) can bind Human EphA3 (RPCB0285) with a linear range of 0.02-2.82 ng/mL. |
