Description
| SKU: | RPCB2800 |
| Size: | 10 μg, 20 μg, 50 μg, 100 μg |
| Tag: | C-6His |
| Reactivity: | Human |
| Protein Description: | Four distinct genes encode alkaline phosphatases (APs) in humans . The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue‑nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers . There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones . The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2,pH 7.5. |
| Gene ID: | 249 |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | NP_000469.3 |
 | Recombinant Human Alkaline phosphatase/tissue-nonspecific isozyme/ALPL Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. Measured by its ability to cleave a fluorogenic substrate, 4- Methylumbelliferyl phosphate (4- MUP). |
 | The specific activity is >29519. 5 pmol/min/µg, as measured under the described conditions. |
