Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
The Rat UCHL1 (Ubiquitin Carboxyl-Terminal Hydrolase L1) ELISA Kit is specifically designed for the accurate quantification of UCHL1 levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for a variety of research applications.UCHL1 is a critical enzyme responsible for regulating protein degradation and maintaining cellular homeostasis. Dysregulation of UCHL1 has been implicated in various diseases, including neurodegenerative disorders and cancer. Therefore, measuring UCHL1 levels can provide valuable insights into the pathogenesis of these conditions and potentially lead to the development of targeted therapies.Overall, the Rat UCHL1 ELISA Kit is an essential tool for researchers investigating the role of UCHL1 in health and disease, offering accurate and precise measurements to advance our understanding of this important enzyme.
Product Name:
Rat UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
SKU:
RTES01096
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
