The Rat LDL-R (LDL Receptor) ELISA Kit is specifically designed to detect levels of LDL-R in rat serum, plasma, and cell culture supernatants with high sensitivity and specificity. This kit provides reliable and reproducible results, making it a valuable tool for researchers in various fields. LDL-R is a key receptor involved in the regulation of cholesterol levels in the body, playing a critical role in the uptake of LDL cholesterol from the bloodstream. Dysregulation of LDL-R has been linked to various diseases, including atherosclerosis and cardiovascular diseases.
Therefore, accurate measurement of LDL-R levels is important for understanding the underlying mechanisms of these conditions and exploring potential therapeutic interventions. With the Rat LDL-R ELISA Kit, researchers can easily quantify LDL-R levels in rat samples, allowing for in-depth studies on cholesterol metabolism and its implications in disease progression. This kit is a valuable asset for any laboratory seeking reliable and accurate measurements of LDL-R levels in rat samples.
Product Name:
Rat LDL R/LDL Receptor ELISA Kit
SKU:
RTFI00274
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-97%
86-103%
86-103%
EDTA Plasma (n = 5)
86-97%
82-99%
86-99%
Heparin Plasma (n = 5)
83-97%
81-99%
81-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
90-92
91
EDTA Plasma (n = 5)
86-104
95
Heparin Plasma (n = 5)
85-105
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
