Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit
The Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit is designed to quantitatively measure the levels of CX3CL1 in various rat biological samples. CX3CL1, also known as Fractalkine, is a unique chemokine that exists in both a membrane-bound form and a soluble form. It plays a critical role as a chemoattractant for leukocytes and is involved in mediating cell adhesion and migration in processes related to inflammation and immune response. Accurate quantification of CX3CL1 levels using this ELISA kit is vital for studying its role in various physiological and pathophysiological processes, including inflammatory responses, immune cell recruitment, and neurological functions. CX3CL1 has been implicated in conditions such as neuroinflammation, immune cell trafficking, and vascular dysfunction, making it a valuable target for research in neuroimmunology, cardiovascular biology, and inflammatory diseases. The Rat CX3CL1 ELISA Kit offered by Assay Genie delivers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under stringent quality standards, this kit provides robust performance and ease of use, making it an ideal choice for researchers conducting investigations into the multifaceted roles of CX3CL1 in biological systems.
Product Name:
Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit
SKU:
AEES00400
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
