The Rat AR (Androgen Receptor) ELISA Kit is specifically developed for the precise quantification of androgen receptor levels in rat samples, including serum, plasma, and cell culture supernatants. With outstanding sensitivity and specificity, this kit provides consistent and trustworthy results, making it an invaluable tool for various research purposes. The androgen receptor is a key protein responsible for mediating the effects of androgens, influencing a variety of biological processes such as development, reproduction, and metabolism. Dysfunction of the androgen receptor has been implicated in various diseases, including prostate cancer and androgen insensitivity syndrome, underscoring its relevance as a potential therapeutic target.
By accurately measuring androgen receptor levels, researchers can gain valuable insights into the role of this protein in health and disease, paving the way for the development of novel treatment strategies. With its user-friendly format and reliable performance, the Rat AR ELISA Kit is an indispensable resource for advancing scientific knowledge in the field of androgen receptor biology.
Product Name:
Rat AR (Androgen receptor) ELISA Kit
SKU:
AEFI00991
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-103%
85-97%
86-97%
EDTA Plasma (n = 5)
85-104%
87-91%
85-100%
Heparin Plasma (n = 5)
88-102%
85-97%
88-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
97-103
100
EDTA Plasma (n = 5)
85-99
90
Heparin Plasma (n = 5)
90-105
99
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
