Rabbit TNF a Recombinant Protein (RPPB0997)

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SKU:
RPPB0997
Product type:
Recombinant Protein
Size:
20ug
Species:
Rabbit
Target:
TNF a
Synonyms:
Tumor necrosis factor
Synonyms:
Cachectin
Synonyms:
TNF-alpha
Synonyms:
Tumor necrosis factor ligand superfamily member 2
Source:
Escherichia Coli
Uniprot:
P04924
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Description

Product Name:Rabbit TNF a Recombinant Protein
Product Code:RPPB0997
Size:20µg
Species:Rabbit
Target:TNF a
Synonyms:Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2. 
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl.
Solubility:It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg
Biological Activity:The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30,000,000 IU/mg.

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E.Coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques.

UniProt Protein Function:Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
UniProt Code:P04924
NCBI GenInfo Identifier:126722916
NCBI Gene ID:100009088
NCBI Accession:NP_001075732.1
UniProt Related Accession:P04924
Molecular Weight:25,817 Da
NCBI Full Name:tumor necrosis factor
NCBI Official Symbol:TNF  
NCBI Official Synonym Symbols:TNFA; TNFSF2  
NCBI Protein Information:tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2
UniProt Protein Name:Tumor necrosis factor
UniProt Synonym Protein Names:Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Protein Family:Tumor necrosis factor
UniProt Gene Name:TNF  
UniProt Entry Name:TNFA_RABIT
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