The Cox2 Antibody (CAB1253) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity specifically with human samples and has been validated for use in Western blot applications. By targeting the PTGS2 protein, this antibody enables the detection and analysis of PTGS2 expression in different cell types, making it suitable for studies in areas such as inflammation, cancer, and pain.PTGS2, a key enzyme involved in the production of prostaglandins, plays a crucial role in inflammatory responses and pain modulation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cox2 Antibody
SKU:
CAB1253
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
PLLP DTFQ IHDQ KYNY QQFI YNNS ILLE HGIT QFVE SFTR QIAG RVAG GRNV PPAV QKVS QASI DQSR QMKY QSFN EYRK RFML KPYE SFEE LTGE KEMS AELE ALYG DIDA VELY PALL VEKP RPDA IFGE TMVE VGAP FSLK GLMG NVIC SPAY WKPS TFGG EVGF QIIN TASI QSLI CNNV KGCP FTSF SVPD PELI KTVT INAS SSRS GLDD INPT VLLK ERST EL
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Purification Method
Affinity purification
Gene ID
5743
RRID
AB_2759370
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using COX2/PTGS2 Rabbit pAb (CAB1253) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using COX2/PTGS2 Rabbit pAb (CAB1253) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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