The PPP1CA Polyclonal Antibody (CAB2184) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PPP1CA protein, this antibody enables precise detection and analysis in a variety of cell types, making it an excellent tool for studies in cell biology and signal transduction pathways.PPP1CA, also known as protein phosphatase 1 catalytic subunit alpha, plays a crucial role in the dephosphorylation of proteins, contributing to the regulation of numerous cellular functions such as cell cycle progression, metabolism, and gene expression.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PPP1CA Polyclonal Antibody
SKU:
CAB2184
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PP1A, PP-1A, PPP1A, PP1alpha, PPP1CA
Positive Sample:
HeLa, NIH/3T3, C6, Mouse liver, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
38kDa
Observed MW:
38kDa
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5499
RRID
AB_2764202
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PPP1CA Rabbit pAb (CAB2184) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using PPP1CA Rabbit pAb (CAB2184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
