The plxIS motif Polyclonal Antibody (CAB24458) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically targeting the PLXIS motif, researchers can gain insights into its role in regulating various cellular processes, making it ideal for studies in cell biology, neuroscience, and cancer research.The PLXIS motif is a conserved sequence found in a variety of proteins involved in intracellular signaling cascades. Understanding the function of this motif is crucial for unraveling complex signaling networks that govern cellular behavior and response to external stimuli.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
plxIS motif Polyclonal Antibody
SKU:
CAB24458
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
293F
Observed MW:
>10kDa
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with plxIS motif using plxIS motif Rabbit pAb (CAB24458) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
