The PIWIL2 Polyclonal Antibody (CAB3073) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to Piwi-like Protein 2, researchers can accurately detect and analyze its presence in various cell types, making this antibody essential for studies in molecular biology and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
PIWIL2 Polyclonal Antibody
SKU:
CAB3073
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CT80, HILI, mili, PIWIL1L, PIWIL2
Positive Sample:
Mouse testis
Cellular Localization:
Cytoplasm.
Calculated MW:
110kDa
Observed MW:
110kDa
PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).
Purification Method
Affinity purification
Gene ID
55124
RRID
AB_2764876
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse testis, using PIWIL2 Rabbit pAb (CAB3073) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
