The PITRM1 Antibody (CAB8362) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it an ideal choice for various research applications.PITRM1 plays a crucial role in maintaining mitochondrial homeostasis by degrading short peptides that target proteins to the mitochondria. Dysregulation of PITRM1 has been linked to neurodegenerative diseases and metabolic disorders, highlighting its importance in cellular function.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PITRM1 Antibody
SKU:
CAB8362
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MP1, PreP, SCAR30, PITRM1
Positive Sample:
U-87MG, NTERA-2
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
117kDa
Observed MW:
117kDa
The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
10531
RRID
AB_2771683
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PITAbGn1 Rabbit pAb (CAB8362) at 1:6600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of L929 cells using PITAbGn1 Rabbit pAb (CAB8362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
