The Phospho-STK3/MST2-T117 Antibody (CABP0934) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and specifically targets the phosphorylation site threonine 117 of STK3. It is highly reactive with human samples and is validated for use in Western blot applications.STK3, also known as MST2, is a serine/threonine kinase that plays a critical role in the Hippo signaling pathway, which controls organ size and tissue homeostasis. Phosphorylation of STK3 at threonine 117 has been linked to its activation and regulation of downstream signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-STK3/MST2-T117 Antibody
SKU:
CABP0934
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
NKTL I
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KRS1, MST2, Phospho-STK3/MST2-T117
Positive Sample:
NIH/3T3
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
56kDa
Observed MW:
61kDa
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6788
RRID
AB_2863846
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STK3/MST2-T117 Rabbit pAb (CABP0934) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
