The Phospho-MET-Y1234/1235 Antibody (CABP0533) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated tyrosine residues Y1234 and Y1235 on the Met protein, this antibody enables precise detection and analysis in a variety of cell types.The Met receptor tyrosine kinase plays a crucial role in signaling pathways involved in cell growth, survival, and motility. Phosphorylation of specific tyrosine residues, such as Y1234 and Y1235, is known to regulate the activation and downstream signaling of Met, making this antibody essential for understanding the mechanisms by which Met activity is controlled.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-MET-Y1234/1235 Antibody
SKU:
CABP0533
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KEYY SV
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
156kDa
Observed MW:
145kDa
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.
Purification Method
Affinity purification
Gene ID
4233
RRID
AB_2771334
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MET-Y1234/1235 Rabbit pAb (CABP0533) at 1:1000 dilution. HeLa cells were treated with HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
