Phospho-CFL1/CFL2 (Tyr88) Antibody (PACO24337)
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주문- SKU:
- PACO24337
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-CFL1/CFL2 (Tyr88) Antibody (PACO24337) |
Antibody SKU: | PACO24337 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse |
Immunogen: | Peptide sequence around phosphorylation site of tyrosine 88 (A-T-Y(p)-E-T) derived from Human coflin1/cofilin2. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from Mouse heart tissue using cofilin1/cofilin2(phospho-Tyr88) Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin1/cofilin2(Phospho-Tyr88) Antibody(left) or the same antibody preincubated with blocking peptide(right). |
Background: | Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. |
Synonyms: | CFL1/CFL2 |
UniProt Protein Function: | Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; intercellular junction; membrane; nuclear matrix; nucleus; vesicle Molecular Function:actin binding; protein binding Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; axon guidance; blood coagulation; cytokinesis after mitosis; cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; establishment of cell polarity; innate immune response; negative regulation of apoptosis; neural crest cell migration; neural fold formation; platelet activation; platelet degranulation; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid stimulus; response to virus; Rho protein signal transduction |
NCBI Summary: | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] |
UniProt Code: | P23528 |
NCBI GenInfo Identifier: | 116848 |
NCBI Gene ID: | 1072 |
NCBI Accession: | P23528.3 |
UniProt Secondary Accession: | P23528,Q53Y87, Q9UCA2, B3KUQ1, |
UniProt Related Accession: | P23528 |
Molecular Weight: | 18,502 Da |
NCBI Full Name: | Cofilin-1 |
NCBI Synonym Full Names: | cofilin 1 |
NCBI Official Symbol: | CFL1 |
NCBI Official Synonym Symbols: | CFL; cofilin; HEL-S-15 |
NCBI Protein Information: | cofilin-1 |
UniProt Protein Name: | Cofilin-1 |
UniProt Synonym Protein Names: | 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform |
Protein Family: | Cofilin |
UniProt Gene Name: | CFL1 |
UniProt Entry Name: | COF1_HUMAN |
Antibodies |
CFL1/CFL2 Antibody (PACO00619) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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