The MRP4/ABCC4 Monoclonal Antibody (CAB22601) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using advanced monoclonal antibody technology, specifically targets the Mrp4/ABCC4 protein, allowing for precise detection and analysis in various experimental settings.Mrp4/ABCC4 is a transporter protein involved in the efflux of a wide range of molecules, including drugs and signaling molecules, from cells. Its overexpression has been linked to chemoresistance in cancer cells, making it a key player in understanding drug resistance mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRP4/ABCC4 Monoclonal Antibody
SKU:
CAB22601
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC52223
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRP4, MOATB, MOAT-B, C4
Positive Sample:
293T, HeLa, HT-29, SW620, LNCaP, Mouse kidney, Rat kidney
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
150kDa
Observed MW:
140-200kDa
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10257
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using [KD Validated] MRP4/ABCC4 Rabbit mAb (CAB22601) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and MRP4/ABCC4 knockdown (KD) 293T cells, using [KD Validated] MRP4/ABCC4 Rabbit mAb (CAB22601) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
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