Description
system_update_altDatasheet
Mouse TGF-beta 1 PharmaGenie ELISA Kit
| Product SKU: | SBRS7957 |
| Size: | 96T |
| Application: | This ELISA antibody pair detects Mouse, Rat and Human TGF-beta 1. Other species not determined. |
| Uniprot: | P04202 |
| Gene ID: | 21803 |
| Gene Names: | Tgfb1 |
| Synonyms: | Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)] |
| Target Species: | Mouse |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 2.46 pg/ml - 600 pg/ml |
| Sensitivity: | 1.5 pg/ml |
| Recommended Dilution: | 60 fold after treatment (see sample activation steps on page 3 of the manual) |
| Intra-Assay CV: | <10% |
| Inter-Assay CV: | <12% |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Mouse TGF-beta 1 Pre-Coated 96-Well Strip Microplate
- Mouse TGF-beta 1 Standard Protein (2 vials)
- Mouse TGF-beta 1 Biotinylated Detection Antibody (2 vials)
- Wash Buffer (20X), 25 ml
- HRP-Streptavidin Concentrate, 200 ul
- TMB One-Step Substrate Reagent, 12 ml
- Stop Solution, 8 ml
- Assay Diluent B (5X), 15 ml
- 96-Well Plate Sealing Film (2 films)
Other materials and equipment required:
The Assay Genie Mouse TGF-beta 1 PharmaGenie ELISA Kit (SBRS7957) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Microplate reader capable of measuring absorbance at 450nm
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Plastic wrap
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT with gentle shaking.
- Add 100 ul of prepared biotinylated detection antibody to each well.
- Incubate 1 h at RT with gentle shaking.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT with gentle shaking.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT in the dark with gentle shaking.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
 | Standard Curve for Mouse TGF-beta 1 PharmaGenie ELISA Kit (SBRS7957) |
