Mouse NT5E (CD73) Recombinant Protein (RPPB4129)

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SKU:
RPPB4129
Product type:
Recombinant Protein
Size:
5ug
Species:
Mouse
Target:
NT5E (CD73)
Synonyms:
5'-nucleotidase (EC:3135)
Synonyms:
5'-NT
Synonyms:
Ecto-5'-nucleotidase
Synonyms:
CD73
Source:
Sf9 Insect cells
Uniprot:
Q61503
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Description

Product Name:Mouse NT5E (CD73) Recombinant Protein
Product Code:RPPB4129
Size:5µg
Species:Mouse
Target:NT5E (CD73)
Synonyms:5'-nucleotidase (EC:3.1.3.5), 5'-NT, Ecto-5'-nucleotidase, CD73, Nt5e, Nt5, Nte.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:NT5E protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDINRKPW NRVVQLEVLC TKCRVPIYEP LEMDKVYKVT LPSYLANGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VVYPAVEGRI KFSLEHHHHH H
Biological Activity:Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25°C.

NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.  

NT5E Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 531 amino acids (29-551a.a.) and having a molecular mass of 59.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).NT5E is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.                                        

UniProt Protein Function:Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
NCBI Summary:This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]
UniProt Code:Q61503
NCBI GenInfo Identifier:13431280
NCBI Gene ID:23959
NCBI Accession:Q61503.2
UniProt Related Accession:Q61503
Molecular Weight:
NCBI Full Name:5'-nucleotidase
NCBI Synonym Full Names:5' nucleotidase, ecto
NCBI Official Symbol:Nt5e  
NCBI Official Synonym Symbols:NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik  
NCBI Protein Information:5'-nucleotidase
UniProt Protein Name:5'-nucleotidase
UniProt Synonym Protein Names:Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name:Nt5e  
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