Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit (AEFI00677)

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SKU:
AEFI00677
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Reactivity:
Mouse
Size:
48T/96T
Detection Method:
Sandwich ELISA, Double Antibody
Sensitivity:
28.125 pg/mL
Range:
46.875-3000 pg/mL
  • Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit (AEFI00677)
  • Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit (AEFI00677)
  • Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit (AEFI00677)

Description

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Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit

The Mouse NQO1 (NAD(P)H Dehydrogenase Quinone 1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of NQO1 levels in mouse serum, plasma, and cell lysates. This kit offers exceptional specificity and precision, ensuring consistent and reproducible results for a variety of experimental applications. NQO1 is a key enzyme involved in the detoxification of quinones and plays a crucial role in cellular antioxidant defense mechanisms. Dysregulation of NQO1 has been linked to oxidative stress, carcinogenesis, and neurodegenerative diseases, highlighting its significance as a biomarker for disease research and therapeutic development.

With the Mouse NQO1 ELISA Kit, researchers can easily quantify NQO1 expression levels in biological samples, allowing for the identification of potential biomarkers and therapeutic targets in various disease states. Its user-friendly format and robust performance make it an essential tool for studying the role of NQO1 in health and disease in mouse models.

Product Name:Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit
SKU:AEFI00677
Reactivity:Mouse
Assay Type:Sandwich ELISA, Double Antibody
Sensitivity:28.125 pg/mL
Range:46.875-3000 pg/mL
Sample Type:Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:2-8°C for 12 months.
Linearity:
Sample1:21:41:8
Serum (n = 5)89-103%85-100%82-100%
EDTA Plasma (n = 5)89-101%84-99%92-95%
Heparin Plasma (n = 5)88-104%82-96%82-93%
Recovery:
SampleRecovery Range (%)Average (%)
Serum (n = 5)87-10195
EDTA Plasma (n = 5)86-9993
Heparin Plasma (n = 5)91-10397
Assay Procedure

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample TypeProtocol
SerumAllow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
PlasmaCollect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue HomogenateHomogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture SupernatantCentrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell LysateLyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample TypesFor more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
ComponentQuantityStorage
48T96T
ELISA Microplate (Dismountable)8×68×12Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard1 vial2 vialPlace the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)60 ul120 ul2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)60 ul120 ul2-8°C (Avoid direct light)
TMB Substrate5 ml10 ml2-8°C (Avoid direct light)
Sample Dilution Buffer10 ml20 ml2-8°C
Antibody Dilution Buffer5 ml10 ml2-8°C
SABC Dilution Buffer5 ml10 ml2-8°C
Stop Solution5 ml10 ml2-8°C
Wash Buffer(25X)15 ml30 ml2-8°C
Plate Sealer3 pieces5 pieces-
Technical Manual1 copy1 copy-

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