The Mouse anti Strep II-Tag mAb (CABE066) is a high-quality antibody developed for reliable detection and analysis of target proteins. The Strep-II tag (WSHPQFEK) is an engineered octapeptide that binds Strep-Tactin with high selectivity under physiological conditions, enabling gentle, one-step affinity purification that preserves protein activity and complex integrity. It is particularly valued in applications requiring native purification conditions, such as protein interaction studies and enzyme activity assays.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Mouse anti Strep II-Tag mAb
SKU:
CABE066
Size:
50μL, 100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0521
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Strep II, Strep II tag, Strep II-tag
Positive Sample:
293T transfected with Strep II-tagged protein (Mouse)
Observed MW:
32kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2863792
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Strep II-tagged protein using Mouse anti Strep II-Tag mAb (CABE066). Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
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