Description
| Product Name: | Mouse Anti-CD11c Low Endotoxin In Vivo Antibody (Phenocycler-Fusion Validated) |
| SKU: | IVMB0285 |
| Size: | 50 µg |
| Antibody Type: | Monoclonal Antibody |
| Clone: | N418 |
| Target: | CD11c |
| Isotype: | IgG |
| Host Species: | Armenian Hamster |
| Reactivity: | Mouse |
| Applications: | IHC FF, PhenoCycler® |
| Synonyms: | αX Integrin, Integrin αX Chain, CR4, p150, ITGAX |
| Product Concentration: | 0.5 mg/ml |
| Immunogen: | Mouse spleen dendritic cells |
| Antigen Distribution: | CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. |
| Formulation: | This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4. |
| Specificity: | Clone N418 recognizes an epitope on mouse CD11C. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or b may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the b2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/b heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the b2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Ligand/Receptor: | iC3b, fibrinogen |
| Product Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
