The Moesin Monoclonal Antibody (CAB4915) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for Moesin in human samples and is suitable for a variety of applications, including Western blot and immunohistochemistry.Moesin is a key regulator of cell shape and movement, playing a role in processes such as cell adhesion, migration, and signaling. Dysregulation of Moesin has been implicated in various diseases, including cancer metastasis and inflammatory disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Moesin Monoclonal Antibody
SKU:
CAB4915
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1258
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KEAL LQAS RDQK KTQE QLAL EMAE LTAR ISQL EMAR QKKE SEAV EWQQ KAQM VQED LEKT RAEL KTAM STPH VAEP AENE QDEQ DENG AEAS ADLR ADAM A
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
Purification Method
Affinity purification
Gene ID
4478
RRID
AB_2863389
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Moesin Rabbit mAb (CAB4915) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using Moesin Rabbit mAb (CAB4915) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
