The Rat ACE2 ELISA (Small Sample Volume) is designed for the quantitative detection of Rat ACE2 levels in various biological samples. Angiotensin-Converting Enzyme (ACE) is a zinc metallopeptidase central to the renin-angiotensin-aldosterone system (RAAS) that catalyzes the conversion of angiotensin I to the potent vasoconstrictor angiotensin II and inactivates the vasodilator bradykinin. ACE is expressed predominantly in pulmonary vascular endothelium and plays critical roles in blood pressure regulation, fluid balance, and vascular tone. Measurement of circulating ACE activity is diagnostically relevant in sarcoidosis, where serum ACE is markedly elevated, and in cardiovascular disease research involving ACE inhibitor pharmacodynamics. Assay Genie's Rat ACE2 ELISA (Small Sample Volume) offers exceptional sensitivity and specificity with a sensitivity of 5.63 ng/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Rat ACE2 ELISA (Small Sample Volume) for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Rat ACE2 ELISA (Small Sample Volume)
SKU:
AEES03490
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
5.63 ng/mL
Detection range:
9.38-600 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
