The Human AMY1 ELISA (Small Sample Volume) is designed for the quantitative detection of Human AMY1 levels in various biological samples. Amylase (AMY) is a family of hydrolytic enzymes that catalyze the breakdown of starch and glycogen to smaller oligosaccharides and monosaccharides. Salivary alpha-amylase (AMY1), produced by parotid and submandibular glands, initiates carbohydrate digestion in the oral cavity, while pancreatic amylase (AMY2) completes starch digestion in the small intestine. Elevated serum amylase activity is a primary diagnostic marker for acute pancreatitis, and its measurement is valuable in the clinical evaluation of pancreatic disease, parotid gland disorders, and salivary biomarker-based stress research. Assay Genie's Human AMY1 ELISA (Small Sample Volume) offers exceptional sensitivity and specificity with a sensitivity of 0.28 ng/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Human AMY1 ELISA (Small Sample Volume) for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human AMY1 ELISA (Small Sample Volume)
SKU:
AEES03416
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
