Description
Maltose Assay Kit (Colorimetric or Fluorometric) (BA0132) (BA0132)
The Maltose Assay Kit (Colorimetric or Fluorometric) (SKU: BA0132) offers a simple, one-step method for measuring maltose in biological and food samples. Maltose is a disaccharide composed of two glucose units linked by an alpha bond, produced from the hydrolysis of glycogen or starch and serving as a source of energy for plants and animals. It can be found in foods such as grains and other processed products. In this assay, maltose is converted to two glucose molecules which are then oxidised to form a coloured product; the colour intensity at 570 nm or fluorescence at 530/585 nm is directly proportional to the maltose concentration in the sample. The procedure is a room-temperature, add-mix-read type assay requiring no 37 degrees C heater. The linear detection range is 2 to 500 uM for colorimetric assays and 1 to 50 uM for fluorometric assays.
| Product Name: | Maltose Assay Kit (Colorimetric or Fluorometric) (BA0132) |
| SKU: | BA0132 |
| Detection Method: | Colorimetric or Fluorometric |
| Detection Range: | 2 to 500 uM (colorimetric); 1 to 50 uM (fluorometric) |
| Sample Type: | Serum, urine, food and beverages |
| Species Reactivity: | All |
| Assay Time: | 60 minutes |
| Kit Size: | 100 Assays |
| Equipment Required: | Microplate reader |
| Storage: | -20 degrees C |
| Shelf Life: | 6 months after receipt |
| Shipping: | Gel Pack |
This kit provides a single-step method for measuring maltose in which maltose is converted to two glucose molecules that are subsequently oxidised to form a coloured, fluorescent product. The colour intensity of the product at 570 nm or fluorescence at 530/585 nm is directly proportional to maltose concentration in the sample.
- Fast and sensitive using 10 uL sample, with a linear detection range of 2 to 500 uM maltose for colorimetric assays and 1 to 50 uM for fluorometric assays
- Convenient single working reagent procedure with absorbance read after 60 minutes at room temperature; no 37 degrees C heater required
- High-throughput add-mix-read type assay that can be readily automated as a 96-well plate assay for thousands of samples per day
- Direct measurement of maltose in various biological samples such as serum, urine, food and beverages
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Prepare 500 uL of 500 uM Premix by mixing 50 uL of the Standard (5 mM) and 450 uL distilled water, then dilute the standards as described in the dilution table. |
| 2 | Transfer 10 uL standards into separate wells of a clear, flat-bottom 96-well plate and transfer 10 uL of each sample into separate wells (each sample requires a sample blank). |
| 3 | Prepare sufficient Working Reagent for all sample and standard wells by mixing, per well, 95 uL Assay Buffer, 1 uL Enzyme A, 1 uL Enzyme Mix and 1 uL Dye Reagent; for the sample blanks prepare a Blank Working Reagent without Enzyme A. |
| 4 | Add 90 uL Working Reagent to the standards and sample wells and 90 uL Blank Working Reagent to the sample blank wells, then tap the plate to mix briefly and thoroughly and incubate 60 minutes at room temperature. |
| 5 | Read optical density at 570 nm (525-605 nm), or for the fluorometric procedure read fluorescence at 530/585 nm using a black 96-well plate. |
Subtract the blank value from the standard values and plot the delta OD against standard concentrations to determine the slope, then calculate [Maltose] = (RSAMPLE - RBLANK) / [Slope (uM-1)] x n (uM), where n is the sample dilution factor. If an internal standard was used, [Maltose] = (RSAMPLE - RBLANK) / (RSTANDARD - RSAMPLE) x [Standard]/2 x n (uM). Conversions: 1 mM maltose equals 34.23 mg/dL or 342.3 ppm.
| Component | Quantity | Storage |
| Assay Buffer | 10 mL | -20 degrees C |
| Enzyme A | 120 uL | -20 degrees C |
| Enzyme Mix | 120 uL | -20 degrees C |
| Dye Reagent | 120 uL | -20 degrees C |
| Standard (5 mM Maltose) | 1 mL | -20 degrees C |
