The LPCAT1 Antibody (CAB4987) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blot and immunofluorescence.LPCAT1 is essential for maintaining cellular membrane integrity and regulating lipid composition, making it a crucial player in processes such as cell proliferation, apoptosis, and lipid metabolism. Dysregulation of LPCAT1 has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LPCAT1 Antibody
SKU:
CAB4987
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Endoplasmic Reticulum Membrane, Golgi Apparatus Membrane, Lipid Droplet, Single-Pass Type Ii Membrane Protein.
Calculated MW:
59kDa
Observed MW:
59kDa
This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers.
Purification Method
Affinity purification
Gene ID
79888
RRID
AB_2765973
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using LPCAT1 Rabbit pAb (CAB4987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of MCF7 cells using LPCAT1 Rabbit pAb (CAB4987) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
