[KO Validated] PFKP Rabbit Polyclonal Antibody (CAB7916)

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SKU:
CAB7916
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
PFKF, PFK-C, PFK-P, ATP-PFK, PFKP
Research Use:
Metabolism

Description

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[KO Validated] PFKP Antibody (CAB7916)

The [KO Validated] PFKP Antibody (CAB7916) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the PFKP protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in metabolism, cancer research, and metabolic diseases.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] PFKP Antibody
SKU:CAB7916
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ATPD TPAC VVSL NGNH AVRL PLME CVQM TQDV QKAM DERR FQDA VRLR GRSF AGNL NTYK RLAI KLPD DQIP KTNC NVAV INVG APAA GMNA AVRS AVRV GIAD GHRM LAIY DGFD GFAK GQIK EIGW TDVG GWTG QGGS ILGT KRVL PGKY LEEI ATQM RTHS INAL LIIG GFEA YLGL LELS AARE KHEE FCVP MVMV PATV S
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:100 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PFKF, PFK-C, PFK-P, ATP-PFK, PFKP
Positive Sample:MCF7, Jurkat, Mouse brain, Mouse heart, Rat testis, Rat brain
Cellular Localization:Cytoplasm.
Calculated MW:86kDa
Observed MW:86kDa

This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID5214
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PFKP Rabbit pAb (CAB7916) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using PFKP Rabbit pAb (CAB7916) (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using PFKP Rabbit pAb (CAB7916) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using PFKP Rabbit pAb (CAB7916) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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