[KO Validated] PKR/EIF2AK2 Rabbit Polyclonal Antibody (CAB5578)

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SKU:
CAB5578
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Synonyms:
PKR, PRKR, DYT33, LEUDEN, EIF2AK1, PPP1R83, PKR/EIF2AK2
Research Use:
Immunology

Description

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[KO Validated] PKR/EIF2AK2 Antibody (CAB5578)

The [KO Validated] PKR/EIF2AK2 Antibody (CAB5578) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for EIF2AK2 in human samples and has been validated for Western blot applications. By binding to the EIF2AK2 protein, this antibody allows for the detection and analysis of EIF2AK2 levels in a variety of cell types, making it a versatile tool for studies in virology and immunology.EIF2AK2, also known as protein kinase R (PKR), is a crucial component of the antiviral response, acting as a sensor for viral infection and initiating the shutdown of protein synthesis to inhibit viral replication.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:[KO Validated] PKR/EIF2AK2 Antibody
SKU:CAB5578
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAGD LSAG FFME ELNT YRQK QGVV LKYQ ELPN SGPP HDRR FTFQ VIID GREF PEGE GRSK KEAK NAAA KLAV EILN KEKK AVSP LLLT TTNS SEGL SMGN YIGL INRI AQKK RLTV NYEQ CASG VHGP EGFH YKCK MGQK EYSI GTGS TKQE AKQL AAKL AYLQ ILSE ETSV KSDY LSSG SFAT TCES QSNS LVTS TLAS ESSS EGDF SADT SEIN SNSD SLNS SSLL MNGL RNNQ RKAK RSLA PRFD LPDM KETK YTVD KRFG MDFK EI
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PKR, PRKR, DYT33, LEUDEN, EIF2AK1, PPP1R83, PKR/EIF2AK2
Positive Sample:HeLa, A-549
Cellular Localization:Cytoplasm, Nucleus, Perinuclear Region.
Calculated MW:62kDa
Observed MW:69kDa

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Purification MethodAffinity purification
Gene ID5610
RRIDAB_2863509
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using PKR/EIF2AK2 Rabbit pAb (CAB5578) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.3s.

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