The IRF9 Polyclonal Antibody (CAB21331) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blot and immunohistochemistry.IRF9, also known as interferon regulatory factor 9, plays a critical role in mediating the antiviral and immune response by forming transcription complexes with other proteins. Its involvement in the regulation of interferon-stimulated genes makes it a crucial player in the immune system's defense against viruses and pathogens.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IRF9 Polyclonal Antibody
SKU:
CAB21331
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p48, IRF-9, ISGF3, ISGF3G, F9
Positive Sample:
A549
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
44kDa
Observed MW:
43kDa
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Mutations in this gene result in Immunodeficiency 65.
Purification Method
Affinity purification
Gene ID
10379
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] IRF9 Rabbit pAb (CAB21331) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from wild type (WT) and IRF9 knockout (KO) A549 cells, using IRF9 Rabbit pAb (CAB21331) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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