Description
IFIH1 Polyclonal Antibody (CAB24868)
The IFIH1 Polyclonal Antibody (CAB24868) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications.IFIH1 is a key player in the innate immune response against viral infections, functioning as a pattern recognition receptor that detects viral nucleic acids.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | IFIH1 Polyclonal Antibody |
| SKU: | CAB24868 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | SNMG SDSG TMGS DSDE ENVA ARAS PEPE LQLR PYQM EVAQ PALE GKNI IICL PTGS GKTR VAVY IAKD HLDK KKKA SEPG KVIV LVNK VLLV EQLF RKEF QPFL KKWY RVIG LSGD TQLK ISFP EVVK SCDI IIST AQIL ENSL LNLE NGED AGVQ LSDF SLII IDEC HHTN KEAV YNNI MRHY LMQK LKNN RLKK ENKP VIPL PQIL GLTA S | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | IFIH1, AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1, interferon induced with helicase C domain 1 |
| Positive Sample: | THP-1 treated with LPS |
| Cellular Localization: | Cytoplasm, Nucleus. |
| Calculated MW: | 25kDa/117kDa |
| Observed MW: | 135kDa |
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19.
| Purification Method | Affinity purification |
| Gene ID | 64135 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from THP-1 cells using MDA5 Rabbit pAb (CAB24868) at 1:1000 dilution. THP-1 cells were treated with LPS (1 μg/ml) at 37℃ for 24 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s. |
