Hypoxia (HIF Pathway) Antibody Sampler Pack

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SKU:
ABSP050
Product Type:
Antibody
Size:
Antibody Sampler Pack
Size:
8 X 20μL
Reactivity:
Human
Mouse
Rat
Analytes:
FIH1 / HIF1AN
HIF-2alpha/EPAS1
VHL
PHD2/EGLN1
CA9
GLUT1/SLC2A1
VEGFA
LDHA
  • Anti-HIF1AN/FIH1 [ARC0826] Monoclonal Antibody -Knockout Validated (CAB3701)
  • Anti-EPAS1/HIF2alpha Antibody (CAB7553)
  • Anti-VHL Antibody (CAB0377)
  • Anti-PHD2 Antibody -Knockout Validated (CAB14557)
  • Anti-Carbonic Anhydrase 9 (CA9/G250) [ARC1275] Monoclonal Antibody (CAB4934)
  • Anti-GLUT1/SLC2A1 [ARC0304] Monoclonal Antibody (CAB11727)
  • Anti-VEGFA Antibody (CAB5708)
  • Anti-LDHA [ARC0509] Monoclonal Antibody (CAB21893)

Description

Hypoxia (HIF Pathway) Antibody Sampler Pack

The Hypoxia (HIF Pathway) Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ELISA, IF/ICC, IHC-P, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.

TargetAntibodyCat. No.HostReactivityApplicationsSize
FIH1 / HIF1ANAnti-HIF1AN/FIH1 [ARC0826] Monoclonal Antibody -Knockout ValidatedCAB3701-20RabbitHumanWBELISA20µL
HIF-2alpha/EPAS1Anti-EPAS1/HIF2alpha AntibodyCAB7553-20RabbitHuman, Mouse, RatWBIHC-PIF/ICCELISA20µL
VHLAnti-VHL AntibodyCAB0377-20RabbitHuman, Mouse, RatWBIHC-PIF/ICCELISA20µL
PHD2/EGLN1Anti-PHD2 Antibody -Knockout ValidatedCAB14557-20RabbitHuman, Mouse, RatWBIHC-PIF/ICCELISA20µL
CA9Anti-Carbonic Anhydrase 9 (CA9/G250) [ARC1275] Monoclonal AntibodyCAB4934-20RabbitHumanWBIHC-PELISA20µL
GLUT1/SLC2A1Anti-GLUT1/SLC2A1 [ARC0304] Monoclonal AntibodyCAB11727-20RabbitHuman, Mouse, RatIHC-PIF/ICCELISA20µL
VEGFAAnti-VEGFA AntibodyCAB5708-20RabbitHuman, Mouse, RatWBIF/ICCELISA20µL
LDHAAnti-LDHA [ARC0509] Monoclonal AntibodyCAB21893-20RabbitHuman, Mouse, RatWBELISA20µL

Cells adapt to low oxygen through the hypoxia-inducible factor pathway, a transcriptional program that reprograms metabolism, angiogenesis, and survival, and that is co-opted in cancer, ischemic disease, and chronic inflammation. The activity of HIF-alpha subunits is controlled primarily at the level of protein stability by oxygen-dependent hydroxylases. Under normoxia, prolyl hydroxylases such as PHD2/EGLN1 use oxygen and 2-oxoglutarate to hydroxylate conserved proline residues on HIF-alpha, creating a recognition motif for the VHL tumor suppressor, the substrate adaptor of an E3 ubiquitin ligase that targets HIF-alpha for proteasomal degradation. A second oxygen sensor, the asparaginyl hydroxylase FIH1/HIF1AN, hydroxylates a residue in the transactivation domain to block coactivator recruitment, providing a graded layer of control. When oxygen is scarce, these hydroxylases are inhibited, HIF-alpha subunits including HIF-2alpha/EPAS1 stabilize, dimerize with their partner, and activate target genes. The resulting transcriptional response promotes anaerobic glycolysis through induction of the glucose transporter GLUT1/SLC2A1 and lactate dehydrogenase LDHA, supports pH regulation via the membrane carbonic anhydrase CA9, and drives angiogenesis through the potent secreted factor VEGFA. This oxygen-tuned stability switch allows the pathway to respond rapidly and proportionately to falling oxygen tension across diverse tissues. In tumors, loss of VHL function or intratumoral hypoxia leads to constitutive HIF activity that fuels metabolic adaptation, vascularization, and aggressive behavior, making this axis an important therapeutic target. Examining FIH1/HIF1AN, HIF-2alpha/EPAS1, VHL, PHD2/EGLN1, CA9, GLUT1/SLC2A1, VEGFA, and LDHA together captures both the oxygen sensing apparatus and the metabolic and angiogenic output it controls. This pack brings together validated antibodies against these key targets to support comprehensive study of the hypoxia response.

AnalyteAlternate Names
FIH1 / HIF1ANHIF1AN, FIH-1, Factor inhibiting HIF-1, FIH1
HIF-2alpha/EPAS1EPAS1, HIF2A, HIF-2-alpha, MOP2, HLF
VHLVon Hippel-Lindau tumor suppressor, VHL1, pVHL
PHD2/EGLN1EGLN1, PHD2, Prolyl hydroxylase domain 2, HPH-2, C1orf12
CA9Carbonic anhydrase 9, CAIX, MN, G250
GLUT1/SLC2A1SLC2A1, GLUT1, GLUT-1, Glucose transporter type 1
VEGFAVascular endothelial growth factor A, VEGF, VPF, VEGF-A
LDHALactate dehydrogenase A, LDH-A, LDHM, PIG19
SpecificationDetail
Pack Format8 antibodies, 20µL each (trial size)
StorageStore at -20°C upon receipt
Shelf Life12 months from date of receipt
ShippingGel pack
Scale-upEvery antibody is also available individually in a full 100µL size

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